Q1.
WHICH PRINCIPLE DOES THE LA PCR KIT VER. 2.1 EMPLOY?
Q2.
IS THERE ANY DIFFERENCE BETWEEN TaKaRa LA Taq AND TaKaRa Ex Taq?
Q3.
WHAT EXACTLY IS THE DIFFERENCE BETWEEN THE BUFFERS ACCOMPANYING
TaKaRa LA Taq AND TaKaRa Ex Taq?
Q4.
WHAT ARE THE RECOMMENDED CYCLING CONDITIONS FOR LA PCR?
Q5.
IS THERE ANYTHING WE SHOULD CONSIDER WHEN SELECTING PRIMERS FOR LA PCR?
Q6.
WHAT IS THE OPTIMAL CONCENTRATION OF PRIMERS FOR LA PCR?
Q7.
WHAT IS THE OPTIMAL CONCENTRATION OF ENZYME FOR LA PCR?
Q8.
HOW SHOULD TEMPLATE DNA BE PREPARED FOR LA PCR?
WHAT AMOUNT OF TEMPLATE DNA SHOULD BE USED?
Q9.
IS IT POSSIBLE TO CARRY OUT LA PCR DIRECTLY ON PHAGE PARTICLES?
Q10.
IS IT POSSIBLE TO DIRECTLY SUBJECT CELL LYSATES OBTAINED BY EITHER
HEAT TREATMENT (98 for 2 minutes) OR PROTEASE DIGESTION FROM EITHER
MAMMALIAN CELLS OR E. coli CELLS TO LA PCR AMPLIFICATION?
Q11.
WHAT CAUSES DIFFUSE SMEARING WITHIN THE LANES UPON ELECTROPHORESIS?
Q12.
WHAT CAUSES NON-SPECIFIC BANDS DETECTED UPON ELECTROPHORESIS?
Q13.
WHAT CAUSES NO OR POOR AMPLIFICATION YIELD?
Q14.
DOES TaKaRa Ex Taq or TaKaRa LA Taq PRODUCE A STICKY END (3O-A OVERHANG)
LIKE TaKaRa Taq?
IS IT POSSIBLE TO USE TA CLONING?
Q15.
WHAT IS THE APPROPRIATE AGAROSE GEL TO USE FOR THE ELECTROPHORESIS
OF LONGER DNA FRAGMENTS GENERATED WITH LA PCR?
AND TaKaRa Ex Taq
PHAGE
PARTICLES?
for 2 minutes) OR PROTEASE DIGESTION FROM EITHER
