Q14.   DOES TaKaRa Ex Taq or TaKaRa LA Taq PRODUCE A

            STICKY END (3'-A OVERHANG) LIKE TaKaRa Taq?

            IS IT POSSIBLE TO USE TA CLONING?

Yes, both TaKaRa Ex Taq and TaKaRa LA Taq generate DNA fragments with 3'-A

overhang that can be cloned into T-vectors.

1 kbp DNA fragment cloned into a vector was used as a template for PCR amplification (conditions shown below) either with TaKaRa LA Taq, TaKaRa Ex Taq or TaKaRa Taq.

The amplified fragments were then cloned into either a T-vector or pUC118/Hinc II and transformed into E. coli JM109. Following cultivation in plates containing IPTG and X-gal,

white colonies were counted for the calculation of A-tailing efficiency.