Primer concentration

The optimal concentration will range from 0.1 to 1.0 . At a lower than optimum concentration,

amplification yield may be poor. On the other hand, at a higher concentration, non-specific

reactions may outperform primer-specific amplifications.

In ordinary practice, primer concentrations can be set depending on the characteristics and amounts of template DNA: low concentrations are recommended either for highly complex DNA such as human genomic DNA, or for high concentrations of template DNA, while high concentrations are preferred for low complexity templates such as plasmid DNA, or for limiting amounts of input template DNA.